ABSTRACT
The different malignancy advancing systems related with focal adhesion kinase (FAK) can be attentive in theprogression of colorectal cancer. By inhibiting the growth mechanism of mixture of 5-Fluorouracil (5-FU) andearthworm therapy could enhance the affectability of malignant growth cells. The objective of this research was toexplore the antiproliferative activity of mixed coelomic fluid cancer medicines produced from Lumbricus rubellus.Colorectal cancer was actuated in mice by injected HT-29 cells in the caecum of mice, and then 5% dextran sodiumsulfate were given by the drinking water. To investigate the effect of combination treatment, we divided of fivetreatment groups: group 1 was untreated (vehicle), groups 2–5 were given by 5-FU. Groups 3–5 were given by acombination of 5-FU (50 μg/g BW) and coelomic fluid (50, 100, and 200 μg/g BW), respectively. The administratedof coelomic fluid was started from the 4th weeks. Mice were sacrificed at the end of 8 weeks and colon tissue wasisolated, then assessed using Flowcytometry and immunofluorescent. These findings revealed that the mixture of 5FUand coelomic fluid components inhibits growth considerably, Interleukin-1β (IL-1β) and FAK compare with 5-FUonly group (p < 0.001; p < 0.05; and p < 0.001). Treatment used coelomic fluid at a dose of 200 μg/g BW decreasedFAK expression. The combination of 5-FU and coelomic fluid at a dose of 200 μg/g BW decreased the percentage ofIL-1β (p < 0.05).
ABSTRACT
Objective To investigate the anti-inflammatory effect of the protein derived from the soluble factor of Heligmosomoides polygyrus (H. polygyrus) excretory-secretory in a colitis model. Methods Colitis was induced by providing drinking water containing 3% dextran sodium sulfate (DSS) for a week. DSS was administrated in a cycle protocol, each cycle consisted of 7 days of 3% DSS in the drinking water and followed by 7 days of regular water. This study consisted of five treatment groups, including Groups A (control) received untreated water, B (DSS only, without excretory-secretory), and C–E injected (i.p.) with excretory-secretory protein (H. polygyrus excretory-secretory total, excretory-secretory 28 kDa and excretory-secretory 55 kDa, respectively). Mice received injection every week. The injection of excretory-secretory was started from the 6th weeks and continued until 11 weeks. At the end of 11 weeks of the experiment, mice were sacrificed, colon tissue was removed and then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, flow cytometry, real-time PCR and histology examination. Results Mice received H. polygyrus excretory-secretory 55 kDa reduced mono-nuclear cell infiltrations. H. polygyrus excretory-secretory 55 kDa induced the down-regulation of mRNA interferon-γ expression. There were significant differences in the expression of mRNA interferon in the colon of mice after the administration of the excretory-secretory 55 kDa protein fraction compared with other groups (P < 0.001), whereas mRNA transforming growth factor-β expression up regulated in the colon of mice after the administration of the excretory-secretory 55 kDa protein fraction compared with total excretory-secretory group (P < 0.05). The treatment of colitis in mice with excretory-secretory 55 kDa protein fractions modulated interleukin-10 (IL-10) expression, whereas excretory-secretory total and excretory-secretory 28 kDa protein fractions insufficient promoted IL-10 expression. Excretory-secretory 55 kDa proteins fraction promoted IL-10 expression via Foxp3-independent pathways. Conclusions Excretory-secretory 55 kDa protein could reduce inflammation and have potential therapy. H. polygyrus excretory-secretory 55 kDa was the soluble factor that may help in the development of novel treatments to cure colitis.